Puerto Chacabuco is a Chilean town in Aisén commune. Administratively it belongs to Aysén Province in Aysén del General Carlos Ibáñez del Campo Region and is located at the head of Aisén Fjord. It is the main port of the region, a port of call for ships sailing to the Laguna San Rafael National Park and the terminus of a Navimag ferry service from Puerto Montt.

Puerto Chacabuco is named after the Battle of Chacabuco in 1817 during the Chilean Independence War.